Hyaluronic acid (HA), also known as hyaluronan, is a naturally occurring glycosaminoglycan (a type of polysaccharide) found throughout the body, particularly in the skin, eyes, joints, and connective tissues. It is renowned for its ability to retain moisture, cushion joints, and support tissue repair.The fermentation liquid was made with glucose as the carbon source and fermented in the culture medium for 48 hours. After the fermentation was completed, the mycelium and impurities were filtered out, and then high-purity products were obtained through simple operations such as alcohol precipitation. Hyaluronic acid produced by fermentation has the advantage of being able to set the molecular weight according to the product design. The key to the fermentation method lies in the selection of the strain, and Streptococcus, Lactococcus, etc. are mostly used.